KMID : 1036720140470030167
|
|
Journal of Nutrition and Health 2014 Volume.47 No. 3 p.167 ~ p.175
|
|
Study of the mechanisms underlying increased glucose absorption in Smilax china L. leaf extract-treated HepG2 cells
|
|
Kang Yun-Hwan
Kim Dae-Jung Kim Kyoung-Kon Lee Sung-Mee Choe Myeon
|
|
Abstract
|
|
|
Purpose: Previous studies have shown that treatment with Smilax china L. leaf extract (SCLE) produces antidiabetic effects due to ¥á-glucosidase inhibition. In this study, we examined the mechanism underlying these antidiabetic effects by examining glucose uptake in HepG2 cells cultured with SCLE.
Methods: Glucose uptake and glucokinase activity were examined using an assay kit. Expression of glucose transporter (GLUT)-2, GLUT-4, and HNF-1¥á was measured by RTPCR or western blot.
Results: Treatment with SCLE resulted in enhanced glucose uptake in HepG2 cells, and this effect was especially pronounced when cells were cultured in an insulin-free medium. SCLE induced an increase in expression of GLUT-2 but not GLUT-4. The increase in the levels of HNF-1¥á, a GLUT-2 transcription factor, in total protein extract and nuclear fraction suggest that the effects of SCLE may occur at the level of GLUT-2 transcription. In addition, by measuring the change in glucokinase activity following SCLE treatment, we confirmed that SCLE stimulates glucose utilization by direct activation of this enzyme.
Conclusion: These results demonstrate that the potential antidiabetic activity of SCLE is due at least in part to stimulation of glucose uptake and an increase in glucokinase activity, and that SCLE-stimulated glucose uptake is mediated through enhancement of GLUT-2 expression by inducing expression of its transcription factor, HNF-1¥á.
|
|
KEYWORD
|
|
Smilax china. L, anti-diabetic activity, glucose uptake, glucose transporter, glucokinase
|
|
FullTexts / Linksout information
|
|
|
|
Listed journal information
|
|
|
|